in vitro and in vivo toxicology services

WuXi Biology is committed to providing in vitro and in vivo toxicology services which support the early drug discovery and lead selection/optimization. In continuation of plate-based efficacy services, we provide rapid and valuable safety evaluation to define the potential of new compounds in your pipeline. Client’s compounds will be quickly analyzed for the safety profile in early stage through most our in vitro HTS in vitro toxicology assay as low as 10ul of 10mM (eg, ~10ul of 10 mM compound can be used for most following in vitro toxicology assays). For further evaluation of compound toxicity, in vivo toxicology study can be initiated and performed in rodents soon in our new SFP level IACUC animal facility.


In vitro toxicology

We provide robust and rapid in vitro toxicity screening approaches to identify compound liabilities and its STR (structure toxicity relationship) in order to further optimize NCE in an early drug discovery stage. Our fast turnaround results from multiple assays and dose-response profiles help our clients to define the potential toxicity of compounds and assist our clients to select lead compounds with higher probability of success and faster speed. Most of our assays are in high throughput format that requires small amounts of test compound with fast data turnaround.

Cytotoxicity

  • CellTiter Glo ATP depletion assay
  • LDH assay
  • MTT assay

Assays are available in HepG2 cells, THLE cells, other hepatocyte, astrocytes, neuron cells, and others upon client request

Cardiotoxicity

  • hERG patch clamp and binding assays
  • Nav1.5 and Cav1.2 patch clamp assay in primary cardiomyocytes
  • Cardiomyocytes Beating Measurement Using Label-free and Real-time RTCA Cardio System

Hepatotoxicity

  • Hepatocyte cell viability
  • Steatosis and phospholipidosis
  • Enzyme induction
  • BSEP Assay

Mitochondrial toxicity

Genotoxicity

  • Ames test (mini and standard Ames)
  • Micronucleus test (CHO/K1)
  • Chromosome aberration assay (CHO/K1)

Drug-drug interaction

  • CYP induction assay
  • P-gp inhibition assay in Caco-2 or MDCK cells

In vivo toxicology studies

WuXi Biology in vivo toxicology services provide flexible access to rodents and non-rodent animal species with a variety of administration routes to characterize adverse effects, determine severity and reversibility, to establish a dose-response relationship, to assist in dose selection for clinical trials and to predict genotoxicity. Clinical observation, histopathology, gross pathology, clinical chemistry, hematology and urinalysis will be evaluated in the following study upon your request. Animal facility is SPF level and AAALAC-compliant for rodent studies.

  • Local tolerance test
  • Dose-range-finding study
  • Acute toxicity study
  • Repeat dose study
  • NOAEL (no observable adverse effect level) determination
  • In vivo micronucleus testing
  • Toxicokinetics