Outstanding Platform to Enable Cancer Immunotherapy with One Stop Service
- Immunological function assessment
- Tumor microenvironment (TME) analysis
- In vivo efficacy evaluation on animal models
- NGS to understand TCR/BCR Repertoire
- Patient TILs analysis
Immuno-Oncology in vitro functional assays
- Immune cell activation assays: T/B/NK cell activation
- Macrophage differentiation assays: M1/M2 macrophage
- DC differentiation
- MDSC induction
- T cell polarization assays: Treg/Th1/Th2/Th17 differentiation
- T cell cytotoxicity assays: Cytotoxic T lymphocyte assay (OT-1 system)
- Mixed Lymphocyte Reaction assays
- Immune cell suppressive assays: MDSC/Treg/Tumor-T suppressive
- Antibody mediated immunological assays: ADCC/CDC/ADCP
- Primary immune cell profiling
Assay | WuXi capibility (Human/Mouse) | |
Call activation | T cell | Yes (H/M) |
B cell | Yes (H) | |
NK cell | Yes (H) | |
Cell differentiation/ polarization | M1/M2 macrophage | Yes (H/M) |
MDSC | Yes (H) | |
DC | Yes (H/M) | |
Treg, Th1, Th2, Th17 polarization | Yes (H) | |
Mixed Lymphocyte Reaction | DC co-culture with T | Yes (H/M) |
Suppressive Assay | MDSC suppressive assay | Yes (H) |
Treg suppressive assay | Yes (H) | |
Tumor cell+immune cell co-culture | Yes (H) | |
Cell cytoxicity | Cytotoxic T lymphocyte assay (OT-1 system) | Yes (M) |
NK cytotoxicity | Yes (H) | |
ADCC, ADCP | Yes (H) | |
CDC | Yes (H) | |
Cell exhaustion | T cell exhaustion | Yes (M) |
Immuno-Oncology in vitro technical expertise
Immune cell profiling: Tumor infiltrating leukocytes (TILs) and other tissues, including spleen blood, lymph node, and liver.
Immune cell population analysis (T, B, NK, DC, Myeloid, Macrophage, Granulocyte, CD4+T, CD8+T, Treg, Th1/2/17, naïve/memory/effector T, g/mMDSC, M1/M2).
Functional and intracellular marker analysis (PD-1, PD-L1/2, CTLA-4, TIM-3, LAG-3, OX40, 4-1BB, ICOS, Ki-67, IL-2, IL-17, IFNγ, TNFα, Granzyme B)
Biomarker
- Soluble biomarker – ELISA, ELISPOT
- Cell-based biomarker – FACS
- Tissue-based biomarker – Multiplex IHC
- Gene expression profiling – RNAseq, NanoString
In Vivo Efficacy Evaluation on Animal Models Syngeneic Murine Models
- Immune-checkpoint humanized models
- Immune-Avatar humanized models PBMC humanized models
- HSC humanized models
Adoptive T Cell Therapy
TM (Tumor Microenvironment) Analysis for Immunoscore by FACS, IHC and IF
CTLs, NK, Tregs, MDSC, TAM, etc.
Lymphoid Tissue and Peripheral Blood Analysis
- Immune cell populations and subsets – frequency, activation, and differentiation
- Cytokine and chemokine analysis
- Anatomical localization of in vivo anti-tumor immune responses
Learn More About Oncology & Immunology
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- OncoWuXi Database