Outstanding Platform to Enable Cancer Immunotherapy with One Stop Service

  • Immunological function assessment
  • Tumor microenvironment analysis
  • In vivo efficacy evaluation on animal models
  • NGS to understand TCR/BCR Repertoire
  • Patient TILs analysis

Immuno-oncology In Vitro Functional Assays

  • Immune cell activation assaysT/B/NK cell activation
  • Macrophage differentiation assaysM1/M2 macrophage differentiation
  • DC differentiation
  • MDSC induction
  • T cell polarization assaysTreg/Th1/Th2/Th17 differentiation
  • T cell cytotoxicity assayCytotoxic T lymphocyte assay (OT-1 system)
  • Mixed Lymphocyte Reaction
  • Immune cell suppressive assayMDSC/Treg/Tumor-T suppressive assay
  • Antibody mediated immunological assayADCC/CDC/ADCP

Assay

WuXi capibility (Human/Mouse)

Call activation

T cell

Yes (H/M)

B cell

Yes (H)

NK cell

Yes (H)

Cell differentiation/

polarization

M1/M2 macrophage

Yes (H/M)

MDSC

Yes (H)

DC

Yes (H/M)

Treg, Th1, Th2, Th17 polarization

Yes (H)

Mixed Lymphocyte Reaction

DC co-culture with T

Yes (H/M)

Suppressive Assay

MDSC suppressive assay

Yes (H)

Treg suppressive assay

Yes (H)

Tumor cell+immune cell co-culture

Yes (H)

Cell cytoxicity

Cytotoxic T lymphocyte assay (OT-1 system)

Yes (M)

NK cytotoxicity

Yes (H)

ADCC, ADCP

Yes (H)

CDC

Yes (H)

Cell exhaustion

T cell exhaustion

Yes (M)

Immuno-oncology In Vitro Technical Competence

Immune cell profiling in tumor infiltrating leukocyte (TIL) and other tissues (spleen, blood, lymph node, liver, etc.) profiling: (1) Immune cell population analysis (T, B, NK, DC, Myeloid, Macrophage, Granulocyte, CD4+T, CD8+T, Treg, Th1/2/17, naïve/memory/effector T, g/mMDSC, M1/M2, etc.) (2) Functional and intracellular marker analysis (PD-1, PD-L1/2, CTLA-4, TIM-3, LAG-3, OX40, 4-1BB, ICOS, Ki-67, IL-2, IL-17, IFNγ, TNFα, Granzyme B, etc.)

Biomarker

  • Soluble biomarker – ELISA, ELISPOT
  • Cell-based biomarker – FACS
  • Tissue-based biomarker – Multiplex IHC
  • Gene expression profiling-RNAseq, Nanostring

In Vivo Efficacy Evaluation on Animal Models Syngeneic Murine Models

  • Immune-checkpoint humanized models
  • Immune-Avatar humanized models PBMC humanized models
  • HSC humanized models

Adoptive T Cell Therapy


TM (Tumor Microenvironment) Analysis for Immunoscore by FACS, IHC and IF

CTLs, NK, Tregs, MDSC, TAM, etc.


Lymphoid Tissue and Peripheral Blood Analysis

  • Immune cell populations and subsets – frequency, activation, and differentiation
  • Cytokine and chemokine analysis
  • Anatomical localization of in vivo anti-tumor immune responses

Learn More About Oncology & Immunology

  • CRISPR/Cas9 Gene Editing
  • Tumor Models
  • Translational Oncology
  • Biomarker
  • Autoimmune and Inflammatory Animal Models
  • OncoWuXi Database