Pharmacology Models for Liver Diseases

Acute and chronic liver diseases are frequent and potentially life threatening for humans. The underlying etiologies are diverse, ranging from viral infections, autoimmune disorders, and intoxications to imbalanced diets and others. Therefore, animal liver disease models are in needs to mimic human liver diseases. WuXi Biology has established multiple animal liver disease models including acute liver injury; obesity related liver damage; nonalcoholic steatohepatitis (NASH); alcoholic steatohepatitis (ASH), liver fibrosis, liver cirrhosis and hepatocellular carcinoma (HCC). These models possess distinct features and etiology of human liver diseases to help evaluate the efficacy of lead compounds in relevant clinical liver diseases and are designed for better understanding of underlying mechanisms for new drugs to be used in clinic.

In vitro biochemistry, cell-based and functional assays

  • MOA and functional analysis including evaluation of cell proliferation, cell apoptosis and specific fibrogenic pathways
  • Characterization of markers involving in liver injury, fibrogenesis or fibrolysis at gene and protein levels with qPCR, ELISA, western blot, LC/MS/MS, etc.
  • Human HSC LX-2, rat HSC T6, primary rodent HSC and hepatocyte systems

In vivo animal models for liver diseases

Acute liver injury models

  • Alcohol induced acute liver injury models
  • CCl4 induced acute liver injury models

Non Alcoholic steatohepatitis (NASH) / HCC models

  • Fatty liver with NASH models in small and large animals (DIO + CCl4 / STZ + HFD)

Complicated factor induced chronic liver injury model

  • High fat diet+alcohol+CCl4 induced liver fibrosis models in small and large animals

Chronic fatty liver models

  • Obesity in liver damage models in small and large animals

Chronic liver injury models

  • Multiple liver fibrosis/cirrhosis models in small and large animals

Endpoint Measurements

  • Clinical observation and physical examination
  • Biomarker analysis
  • Histology analysis

Fibrosis Models

Animal Models:

  • Liver: CCL4-HFD, CCL4, ANIT induced fibrosis
  • Lung: Idiopathic pulmonary fibrosis
  • Kidney: UUO induced fibrosis Diabetic induced fibrosis

Measurements:

  • Biomarker, histology and scoring fibrosis