CNS Animal Models

CNS animal models for neurodegenerative diseases; Alzheimer's, Parkinson's, Huntington's

WuXi Biology offers a full range of CNS efficacy and safety pharmacology assessments, from basic models and regulatory tests, to the evaluation of abuse and dependence liability and proconvulsant risk using EEG.

Our experts provide animal models in all areas of psychopharmacology, cognition, epilepsy and neurodegenerative disorders.


in vivo models for Neurodegenerative Diseases

Alzheimer’s disease (AD) models

Alzheimer's disease models; beta-amyloid, FAD transgenic mouse, STZ induction, AAP/PS1
  • Genetically modeling in the mouse: Expression of a human mutant gene or a BAC fragment that is associated with a familial type of AD
  • Pharmacologically/neurosurgically modeling in the rat: Ventricular infusion of β-amyloid with an Osmotic mini pump

IN VIVO PHARMACOLOGY CATALOG | AD

NEU-A01-1Alzheimer’s Disease ModelAAP/PS1 transgenic mouse
NEU-A01-2Alzheimer’s Disease ModelAAPSWE transgenic mouse
NEU-A01-3Alzheimer’s Disease Model5x FAD transgenic mouse
NEU-A01-4Alzheimer’s Disease ModelStreptozotocin (STZ) induction, sporadic
NEU-A01-5Alzheimer’s Disease ModelNeurotoxin (phencyclidine-PCP or cisplatin) induction
NEU-A01-6Alzheimer’s Disease ModelGoto-Kakizaki (GK) type 2 diabetes rat

Parkinson’s Disease (PD) models

Parkinson's disease models, 6-OHDA induction, MPTP, PDD, dementia, neuronal loss
  • Genetic model in the mouse: Expression of a mutant gene that is associated with a familial autosomal-dominant mutation, or knockout of a gene that is associated with a familial autosomal-recessive mutation for PD
  • Pharmacological / Neurosurgical models
    • Systemic administration of MTPT to lesion dopamine neurons in a NHP (under development)
  • Behavioral neurology Parkinson’s Disease Dementia (PDD) Model

IN VIVO PHARMACOLOGY CATALOG | PD

NEU-A02-1Parkinson’s Disease ModelUnilateral intra-striatal injection of 6-hydroxydopamine (6-OHDA) to lesion nigro-striatal dopamine neurons
NEU-A02-2Parkinson’s Disease Model
| moderate to severe DA neuronal loss
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induction
NEU-A02-3Parkinson’s Disease ModelAAV alpha-synuclein overexpression/preformed fibril (PFF)
NEU-C02-1Parkinson’s Disease Dementia (PDD) Mode6-hydroxydopamine (6-OHDA) induction

Huntington’s Disease model

Huntington's disease models, transgenic mouse, quinolinic acid, Huntington mutant gene
  • Genetic model in the mouse: Expression of a mutant Huntington gene
  • Pharmacological/neurosurgical model

IN VIVO PHARMACOLOGY CATALOG | HD

NEU-A03-1Huntington’s Disease ModelTrangenic model in the mouse
NEU-A03-2Huntington’s Disease ModelStriatal microinjection of quinolinic acid or 3-NY in the rat

Multiple Sclerosis Model

Experimental Autoimmune Encephalomyelitis, MOG-EAE, multiple sclerosis, cuprizone model

Endpoints

  • MOG-induced EAE: clinical score, incidence, and inflammation response
  • MBP-induced EAE: body weight, clinical score, and incidence rate
  • Cuprizone model: grip test, ledged beam test, and myelin staining

IN VIVO PHARMACOLOGY CATALOG | MS

NEU-A04-1Multiple Sclerosis Model | Chronic ModelMyelin Oligodendrocyte Glycoprotein (MOG35-55 peptide) induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 mice
NEU-A04-2Multiple Sclerosis ModelMyelin Basic Protein (MBP68-86 peptide) induced EAE in Lewis rats  
NEU-A04-3Multiple Sclerosis ModelCuprizone intoxication model

Age-dependent Neurodegeneration

Neurofibrillary tangle-like structure, dementia, PS1/PS2 knockout, tau hyper-phosphorylation

Conditional double knockout PS1/PS2 in the mouse: which shows age-dependent neuronal loss and many other AD-like pathologies such as tau hyperphosphorylation, neurofibrillary tangle-like structure, and dementia


Neurology

Neurology, neurobiology models, including seizure and dementia, autism, stroke, ischemia

Seizure Models

Dementia models

Autism

Stroke Models

  • Middle cerebral artery occlusion (MCAO) model in rats
  • MCAO reperfusion model in rats

Ischemia model

  • Forebrain ischemia model in (rat and gerbil)

Traumatic Brain Injury

BEHAVIORAL NEUROLOGY CATALOG

NEU-C01-1Seizure ModelAcute/chronic restraint stress (ARS/CRS)
NEU-C01-2Seizure ModelLearned helplessness (LH)
NEU-C01-3Seizure ModelChronic corticosterone (CORT) induction
NEU-C01-4Seizure ModelChronic social defeat stress (CSDS)
NEU-C02-1Parkinson’s Disease Dementia (PDD) Model6-hydroxydopamine (6-OHDA) induction
NEU-C03-1Autism spectrum disorder (ASD) ModelShank +/-
NEU-C04-1Ischemic Strokemiddle cerebral artery occlusion MCAO
NEU-C04-2Forebrain Ischemia Modelfour-vessel occlusion (4VO)
NEU-C05-1Traumatic Brain Injuryby external force

Psychopharmacology | Mental Disorder Models

NEUROPSYCHIATRIC, Psychopharmacology models, depression, stress

By combining experience and expertise from both the pharmaceutical industry and academia, we provide valuable services to accelerate drug discovery for the treatment of psychiatrics diseases and mental disorders.

Depression

  • Depression Models
  • Depression-like behavior
  • Behavioral induction
  • Chemical induction

NEUROPSYCHIATRIC IN VIVO CATALOG

NEU-C01-1Depression ModelAcute/chronic restraint stress (ARS/CRS)
NEU-C01-2Depression ModelLearned helplessness (LH)
NEU-C01-3Depression ModelChronic corticosterone (CORT) induction
NEU-C01-4Depression ModelChronic social defeat stress (CSDS)
NEU-C01-5Depression ModelChronic unpredictable mild stress (CUMS)
NEU-T03-5Behavioral TestTail Suspension Test (TST)
NEU-T03-6Behavioral TestForced Swim Test (FST)
NEU-T03-7Behavioral TestSocial Interaction Test (SIT)
NEU-T03-8Behavioral TestSources Preference Test (SPT)

Anxiety

  • Genetic model in the mouse: Overexpression of the cholecystokinin receptor-2 (CCKR-2) in the forebrain
  • Various stress paradigms: early-life stress, chronic stress, or acute stress
  • PTSD model in the mouse: combination of genetic manipulation and environmental stress
NEU-T03-1Behavioral Test
Anxiety
Elevated Plus Maze Test (EPM)
NEU-T03-2Behavioral Test
Anxiety
Vogel Test
NEU-T03-3Behavioral Test
Anxiety
Open Field Test (OFT)
NEU-T03-4Behavioral Test
Anxiety
Novelty Suppressed Feeding (NSF)

Schizophrenia

Bipolar disorder

  • Experimental Psychiatry
  • Behavioral induction
  • Chemical induction

Mouse | Rat

NEU-C02-1Schizophrenia ModelDrug-induced hyperlocomotion, positive symptoms
NEU-C02-2Schizophrenia Model Acute restraint stress (ARS), negative symptoms
NEU-C02-3Schizophrenia ModelChronic corticosterone (CORT) induction, negative symptoms
NEU-T03-9Behavioral Test | Anti SchizophrenicConditioned Avoidance Response (CAR)
NEU-T03-10Behavioral Test | Schizophrenia, cognitivePre-pulse Inhibition (PPI)
NEU-T03-11Behavioral Test | Schizophrenia, cognitive Attentional Set-Shifting Test (ED/ID test)
NEU-T03-12Behavioral Test | Schizophrenia, negative3-Chamber Social Interaction Test

Addiction

Drug addiction behavior models, sensitization, CPP, conditioned place preference
  • Behavioral sensitization
  • Drug-seeking behavior
  • Behavioral induction
  • Chemical & behavioral induction

Mouse | Rat

DRUG ADDICTIVE BEHAVIOR | IN VIVO CATALOG

NEU-E01-1Addiction ModelConditioned place preference (CPP)
NEU-E01-2Addiction ModelSelf-administration (SA)
NEU-E01-3Addiction ModelBehavioral sensitization (BS)

Neurosensory Diseases

Neurosensory disease models, ocular degeneration, vestibular dysfunction, hearing loss

Hearing Loss and Vestibular Dysfunction

Hearing Loss

Ocular Degeneration

  • Surgery & chemical
  • Physical induction
  • Transgenic model

Mouse | Rat | Guinea pig

NEUROSENSORY DISEASES MODELS | IN VIVO CATALOG

NEU-F01-1Hearing Loss & Vestibular Dysfunction ModelAminoglycoside antibiotics induction
NEU-F01-2Hearing Loss & Vestibular Dysfunction ModelSelf-administration (SA)
NEU-F02-1Hearing Loss Model3,3′-Iminodipropionitrile (IDPN) induction
NEU-F02-4Hearing Loss ModelNoise induction
NEU-F03-1Rod and Cone Degeneration ModelAged rodless/coneless transgenic mouse

Motor Neuron Diseases

Amyotrophic lateral sclerosis, motor neuron diseases, SOD1G93A transgenic rat, ALS

Amyotrophic Lateral Sclerosis

NEU-G01-1Amyotrophic lateral sclerosis (ALS) ModelSOD1G93A transgenic rat

Insomnia

Insomnia model, caffeine induction, surgery and surgical induction

Amyotrophic Lateral Sclerosis

  • Chemical induction
  • Surgery

Mouse | Rat

NEU-H01-1Insomnia ModelCaffeine induction
NEU-H01-2Insomnia ModelPost-surgery

Electrophysiology

ELECTROPHYSIOLOGICAL TESTS

Electrophysiology tests, EEG, ECG, EMG, telemetry, nerve conduction velocity, evoke potential

Electrogram

Evoke Potential

Nerve Conduction Velocity

Auditory Brainstem Response

Vestibule-Ocular Reflex

Telemetry

Muscle Strength Test

Ex Vivo Test

NEU-U01-1ElelctrogramElectroencephalogram (EEG)
NEU-U01-2ElelctrogramElectrocardiogram (ECG)
NEU-U01-3ElelctrogramElectromyogram (EMG)
NEU-U01-4ElelctrogramSleeping Architecture Analysis
NEU-U02-1Evoke PotentialVisual Evoked Potential (VEP)
NEU-U02-1Evoke PotentialEvoked Response of Wide Dynamic Range (WDR) Neuron
NEU-U03-1Nerve Conduction VelocityNerve Conduction Velocity Test
NEU-U04-1Auditory Brainstem Response Auditory Brainstem Response (ABR)
NEU-U05-1Vestibule-Ocular ReflexVestibule-Ocular Reflex (VOR)
NEU-U06-1TelemetryTelemetry of Blood Pressure
NEU-U06-2TelemetryTelemetry of Body Temperature
NEU-U06-3TelemetryTelemetry of Spontaneous Activity 
NEU-U07-1Muscle Strength Muscle Strength Test
NEU-U08-1Ex Vivo TestLangendorff Test of Perfused Heart
NEU-U08-2Ex Vivo TestMuscle Contraction Test

Behavioral Studies

Behavioral studies, Morris water maze, rotarod test, NOR, novel object recognition, grip strength

Learning and memory

  • Morris water maze
    • Spatial learning and memory, working memory, reference memory, reversal learning, hippocampus-dependent memory
  • Barnes maze
    • Spatial learning and contextual conditioning (hippocampus-dependent)
    • Cued conditioning (hippocampus-independent)
    • Long-term memory, short-term memory, amygdala-dependent memory, fear response

Motor function

  • Rotarod test
  • Open-field test
  • Walking-beam test
  • Treadmill test (under development)

Anxiety-like behavior

  • Elevated-plus maze
  • Fear-conditioning test
  • Open-field behavior
  • Startle response
  • Social interaction

Depression-like behavior

  • Novelty-induced hypophagia
  • Tail suspension test
  • Forced swim test
  • Open-field behavior
  • Social interaction
  • Learned helplessness

Schizophrenia/bipolar-like behavior

  • Working memory
  • Locomotion/motor activity
  • Social interaction
  • Aggressive/mania-like behavior
  • Forced swim test
  • Nesting behavior
  • Pre-pulse inhibition (PPI) under development

Home cage behavior and environmental enrichment

Pain-related behavior

  • Hot-plate
  • Tail flick (infrared heat/pressure)

BEHAVIORAL TESTS

NEU-T01-1Cognitive BehaviorNovel Object Recognition (NOR)
NEU-T01-2Cognitive BehaviorFear Conditioning
NEU-T01-3Cognitive BehaviorMorris Water Maze
NEU-T01-4Cognitive BehaviorObject Location Memory Task
NEU-T01-5Cognitive BehaviorPassive Avoidance Task
NEU-T01-6Cognitive BehaviorT Maze
NEU-T01-7Cognitive BehaviorY Maze
NEU-T01-8Cognitive BehaviorTraining box
NEU-T02-1Sensorimotor BehaviorActivity Chamber
NEU-T02-2Sensorimotor BehaviorBasket Test
NEU-T02-3Sensorimotor BehaviorCylinder Test
NEU-T02-4Sensorimotor BehaviorGrip Strength
NEU-T02-5Sensorimotor BehaviorHot Plate
NEU-T02-6Sensorimotor BehaviorLedged Beam Test
NEU-T02-7Sensorimotor BehaviorNeurological Testing
NEU-T02-8Sensorimotor BehaviorRotor-Rod
NEU-T02-9Sensorimotor BehaviorWire Hang Test
NEU-T02-10Sensorimotor BehaviorBalance Beam Test
NEU-T02-11Sensorimotor BehaviorOlfactory Test
NEU-T02-12Sensorimotor BehaviorIrwin Test
NEU-T02-13Sensorimotor BehaviorVisual Placing Response
NEU-T02-14Sensorimotor BehaviorVisual Water Maze
NEU-T02-15Sensorimotor BehaviorVisual Cliff Test
NEU-T03-1Experimental Psychiatry Anxiety | Elevated Plus Maze Test (EPM)
NEU-T03-2Experimental PsychiatryAnxiety | Vogel Test
NEU-T03-3Experimental PsychiatryAnxiety | Open Field Test (OFT)
NEU-T03-4Experimental PsychiatryAnxiety| Novelty Suppressed Feeding (NSF)
NEU-T03-5Experimental PsychiatryDepression | Tail Suspension Test (TST)
NEU-T03-6Experimental PsychiatryDepression | Forced Swim Test (FST)
NEU-T03-7Experimental PsychiatryDepression | Social Interaction Test (SIT)
NEU-T03-8Experimental PsychiatryDepression | Sources Preference Test (SPT)
NEU-T03-9Experimental PsychiatryAnti Schizophrenic | Conditioned Avoidance Response (CAR)
NEU-T03-10Experimental PsychiatrySchizophrenia, cognitive | Pre-pulse Inhibition (PPI)
NEU-T03-11Experimental PsychiatrySchizophrenia, cognitive | Attentional Set-Shifting Test (ED/ID test)
NEU-T03-12Experimental PsychiatrySchizophrenia, negative | 3-Chamber Social Interaction Test
NEU-T03-13Experimental PsychiatryObsessive-Compulsive Disorder | Marble Burying Test
NEU-T03-14Experimental PsychiatrySocial Behavior | 3-Chamber Sociability and Social Novelty Test
NEU-T03-15Experimental PsychiatrySocial Behavior | Tube Dominance Test