High Throughput Screening

WuXi Biology offers high-throughput screening (HTS) services in drug discovery to identify new chemical matter. Our experts provide high-quality and cost-effective screening services covering biochemical, radiometric, and cell-based, to high content, multiplex, LC/MS based screening for hit identification and lead discovery.

In addition, we provide compound profiling services. Pharmacology profiling has become an invaluable tool for lead compound selection, optimization and candidate prioritization. Analysis of profile data identifies off-target interactions that can help predict potential side-effects, efficacy and safety of compounds under development before they progress to the more costly stages of preclinical and clinical trials. WuXi Biology offers comprehensive profiling services to fit our clients’ drug discovery & development needs.

HTS Compound Collection

  • Majority of the collection is semi-exclusive, non-commercially available
  • Selected from >550K compounds based on diversity, properties, PAINS* & Vacca-Reos# structural filters, reviewed by medicinal chemists, and Rule-of-5 Multi Parameter Optimization (MPO)
  • Total 167k compounds were collected and another 50k compounds expansion in progress
  • Arrayed in 384 plate ready for HTS

HTS Collection (166K) – Property Distributions

HTS Compound Collection, high throughput screening, 384-plate array

WuXi HTS Case Study

  • Over 40 HTS successfully completed with WuXi HTS library.
  • Multiple HTS projects have successfully identified hits for further lead identification and optimization.
  • Targets cover diverse fields
    • Epigenetic targets
    • GPCRs and nuclear receptors
    • Kinases
  • Example of primary screening QC data
    • Z’ value of plates in primary screening
    • IC50 result of reference compound in cherry picking screening
Z prime value of HTS plates from primary screening, IC50 values from reference, control compounds

State-of-the-art Equipment for HTS Support

We regularly invests in the best HTS screening platform technologies to support our clients needs in screening activities:

  • Automation system
    • Tecan
    • POD and Echo
    • JANUS
    • BRAVO
    • Platemate Plus
  • Readers to support HTS
    • BD Biosciences Flow Cytometer with HTS mode
    • Envision (with stacker)
    • EnSpire2300
    • FlexStation III
    • Bioplex 200 Luminex
    • FLIPR and Hamammatsu
    • Topcount and Microbeta2
    • Q-Patch and Ion Works
    • Acumen and Odyssey (Li-COR)
  • Plate washers
    • Elx405 and Elx406
    • DropArray Washing StationPlate Sealers