In Vitro Screening Platform
WuXi Biology offers screening services to identify new chemical matter. Our experts provide high-quality and cost-effective screening services covering biochemical, radiometric, and cell-based, to high content, multiplex, LC/MS based screening for hit identification and lead discovery. In addition, we provide compound profiling services.
Pharmacology profiling has become an invaluable tool for lead compound selection, optimization and candidate prioritization. Analysis of profile data identifies off-target interactions that can help predict potential side-effects, efficacy and safety of compounds under development before they progress to the more costly stages of preclinical and clinical trials. WuXi Biology offers comprehensive profiling services to fit our clients’ drug discovery & development needs.
High Throughput Screening
HTS Compound Collection
- Majority of the collection is semi-exclusive, non-commercially available
- Selected from >550K compounds based on diversity, properties, PAINS* & Vacca-Reos# structural filters, reviewed by medicinal chemists, and Rule-of-5 Multi Parameter Optimization (MPO)
- Total 167k compounds were collected and another 50k compounds expansion in progress
- Arrayed in 384 plate ready for HTS
HTS Collection (166K) – Property Distributions
High Throughput Screening Case Study
- Over 40 HTS successfully completed with WuXi HTS library.
- Multiple HTS projects have successfully identified hits for further lead identification and optimization.
- Targets cover diverse fields
- Epigenetic targets
- GPCRs and nuclear receptors
- Kinases
- Z’ value of plates in primary screening
- IC50 result of reference compound in cherry picking screening
Example of primary screening QC data
State-of-the-art Equipment for HTS Support
We regularly invests in the best HTS screening platform technologies to support our clients needs in screening activities:
- Automation system
- Tecan
- POD and Echo
- JANUS
- BRAVO
- Platemate Plus
- lx405 and Elx406
- DropArray Washing StationPlate Sealers
- Readers to support HTS
- BD Biosciences Flow Cytometer with HTS mode
- Envision (with stacker)
- EnSpire2300
- FlexStation III
- Bioplex 200 Luminex
- FLIPR and Hamammatsu
- Topcount and Microbeta2
- Q-Patch and Ion Works
- Acumen and Odyssey (Li-COR)
High Content Platform
Cell-based functional bioassays of bio-relevant activities of enzymes, receptors, and signaling molecules and pathways. Integrated multi-parameter analysis for customized specific needs.
Applications
- Targeted Drug Screening
- Target-Hit Search
- Hit to Lead Identification
- Lead Optimization
- Drug Mechanism of Actions
- Drug Kinetics
- Drug Cytotoxicity
Specific Assays: 2D/3D/4D Image Based Quantitative and Semi-Quantitative Functional Bioassays
- Cell Proliferation Assays (growth rate, doubling time, viability)
- Cell Death Assays (apoptosis, necrosis, autophagy)
- Cell Cycle Assays (cell cycle phases, mitosis, quiescence, senescence)
- Subcellular Localization & Translocation Assays (endocytosis, exocytosis, nuclear/cytosol)
- Subcellular Organelle Functional Assays (mitochondria, Golgi, endoplasmic reticulum)
- Membrane Integrity and Functional Analysis
- Kinase and Signal Transduction Analysis
- Cytoskeleton and Extracellular Matrix Analysis
- Cell Motility, Invasion, and Chemotaxis Assay
- Oxidative Stress and Cellular Response Analysis
- DNA and Chromosome Damage Analysis
- Transcription Activation and Gene Expression Analysis
- DNA/Protein Synthesis and Degradation Analysis
- Protein-Protein, Protein-DNA, Protein-RNA Interaction Analysis
- Customized Services – Please contact us for your special needs.
