Target-to-Hit Services

Successful medicine begins with target validation and target discovery. Finding druggable targets remains pivotal to the future R&D success of pharmaceutical companies. WuXi Biology’s innovative platform serves clients in the identification and validation of both known and novel targets to understand their contexts of diseases and accelerate go versus no-go decisions thus reducing the risks that impact clients’ drug R&D portfolios.

As one of WuXi AppTec’s well recognized strengths, our target validation program has teamed with several major pharmaceutical companies for years to support their programs from oncology, metabolic diseases, and translational medicine, to pharmacogenomics. This has resulted in more than 50 various potential drug targets delivered to our clients.

Drug Target Validation

  • Target knockdown, co-knockdown of isoforms, and target overexpression
  • Conventional knockout, knock-in; conditional regional, organ, or cell type specific knockout in mice or isogenic cell lines
  • Region specific or cell type specific inducible/reversible transgenic mice
  • BAC/YAC transgenic mice for humanized animal models
  • Evaluation of target functional roles
  • Characterization of target activities & correlations in cell lines with positive and negative effects
  • Rescue study for target specificity confirmation

Target Engagement and MOA

  • Pathway dissecting & phenotypic study
  • In vitro functional readout & evaluation
  • MOA & signaling cascade
  • Targeting specific tissues for MOA study
  • In vivo phenotypic characterizations in various disease models
  • shRNA or sgRNA library screening for target identification
  • IHC, FACS, WB, qPCR for target engagement

Translational Predictive Biomarkers

  • Multiple marker classes including DNA, RNA, protein & microRNA markers
  •  in vitro assays & in vivo disease models
  • Assay development
  • Bioinformatics supports