2D/3D Cell Culture

Cell Engineering Service

  • Gene knock-out, allele-specific genome editing, and inducible gene knock-down
  • Drug resistant cell line generation, stable cell line generation, phenotypic analysis (key technology: CRISPR/Cas9, lentiviral system)

CRISPR/Cas9 Based Method for KO/KI Cell Line Generation

Target-based Mechanistic Assays

  • DNA, RNA, protein level
  • Subcellular localization
  • Signaling pathway research
  • Cellular detection of protein phosphorylation
  • Protein-Protein interaction
  • Cell metabolite detection
  • Cell morphology and phenotype analysis

Potency Correlation of Protein-Protein Interaction

Cell Function

  • Cell viability/toxicity
  • Cell proliferation
  • Cell cycle
  • Cell motility/migration
  • Cell apoptosis
  • 2D/3D Clonogenic assay

OrisTM Migration Assay

Transwell Migration Assay

3D Clonogenic Assay

Outstanding Platform to Enable Cancer Immunotherapy with One-Stop Service

  • Immunological function assessment
  • Tumor microenvironment analysis
  • In vivo efficacy evaluation on animal models
  • NGS to understand TCR/BCR repertoire
  • Patient TILs analysis

Immuno-oncology In Vitro Functional Assays

  • Immune cell activation assays
    • T/B/NK cell activation
  • Macrophage differentiation assays
    • M1/M2 macrophage differentiation
    • DC differentiation
    • MDSC induction
  • T cell polarization assays
    • Treg/Th1/Th2/Th17 differentiation
  • T cell cytotoxicity assay
    • Cytotoxic T lymphocyte assay (OT-1 system)
  • Mixed lymphocyte reaction
  • Immune cell suppressive assay
    • MDSC/Treg/Tumor-T suppressive assay
  • Antibody mediated immunological assay


WuXi capibility (Human/Mouse)

Call activation

T cell

Yes (H/M)

B cell

Yes (H)

NK cell

Yes (H)

Cell differentiation/


M1/M2 macrophage

Yes (H/M)


Yes (H)


Yes (H/M)

Treg, Th1, Th2, Th17 polarization

Yes (H)

Mixed Lymphocyte Reaction

DC co-culture with T

Yes (H/M)

Suppressive Assay

MDSC suppressive assay

Yes (H)

Treg suppressive assay

Yes (H)

Tumor cell+immune cell co-culture

Yes (H)

Cell cytoxicity

Cytotoxic T lymphocyte assay (OT-1 system)

Yes (M)

NK cytotoxicity

Yes (H)


Yes (H)


Yes (H)

Cell exhaustion

T cell exhaustion

Yes (M)

Immuno-oncology In Vitro Technical Competence 

Immune cell profiling in tumor infiltrating leukocyte (TIL) and other tissues (spleen, blood, lymph node, liver, etc.) profiling:

  1. Immune cell population analysis (T, B, NK, DC, Myeloid, Macrophage, Granulocyte, CD4+T, CD8+T, Treg, Th1/2/17, naïve/memory/effector T, g/mMDSC, M1/M2, etc.)
  2. Functional and intracellular marker analysis (PD-1, PD-L1/2, CTLA-4, TIM-3, LAG-3, OX40, 4-1BB, ICOS, Ki-67, IL-2, IL-17, IFNγ, TNFα, Granzyme B, etc.)


  • Soluble biomarker – ELISA, ELISPOT
  • Cell-based biomarker – FACS
  • Tissue-based biomarker – Multiplex IHC
  • Gene expression profiling-RNAseq, Nanostring

Tumor Infiltrating Lymphocytes (TILs) Analysis on Human and Murine Tumor Tissues

  • CD4, CD8, MDSC, M1/M2, DC cell, NK cell, B cell
  • PD1, TIM3, LAG3, OX40, 4-1BB
  • Treg, Th1,2,3,17 …

In Vivo Efficacy Evaluation on Animal Models Syngeneic Murine Models

  • Immune-checkpoint humanized models
  • Immune-Avatar humanized models PBMC humanized models
  • HSC humanized models

TM (Tumor Microenvironment) Analysis for Immunoscore by FACS, IHC and IF

CTLs, NK, Tregs, MDSC, TAM, etc.

Lymphoid Tissue and Peripheral Blood Analysis

  • Immune cell populations and subsets – frequency, activation, and differentiation
  • Cytokine and chemokine analysis
  • Anatomical localization ofin vivo anti-tumor immune responses

High Content Platform


Cell-based functional bioassays of bio-relevant activities of enzymes, receptors, and signaling molecules and pathways. Integrated multi-parameter analysis for customized specific needs.


  • Targeted Drug Screening
  • Target-Hit Search
  • Hit to Lead Identification
  • Lead Optimization
  • Drug Mechanism of Actions
  • Drug Kinetics
  • Drug Cytotoxicity

Specific Assays: 2D/3D/4D Image Based Quantitative and Semi-Quantitative Functional Bioassays

  • Cell Proliferation Assay (growth rate, doubling time, viability, etc.)
  • Cell Death Assay (apoptosis, necrosis, autophagy)
  • Cell Cycle Assay (cell cycle phases, mitosis, quiescence, senescence)
  • Subcellular Localization & Translocation Assay (endocytosis, exocytosis, nuclear/cytosol, nuclear structure)
  • Subcellular Organelle Function Assay (mitochondria, Golgi body, endoplasmic reticulum, etc.)
  • Membrane Integrity and Function Analysis
  • Kinase and Signal Transduction Analysis
  • Cell-Cell Interaction Analysis
  • Cell Sub-Population Analysis and Enrichment
  • Cytoskeleton and Extracellular Matrix Analysis
  • Cell Motility, Invasion, and Chemotaxis Assay
  • Oxidative Stress and Cellular Response Analysis
  • DNA and Chromosome Damage, and Genetic Instability Analysis
  • Epigenetic Modification Analysis
  • Transcription Activation and Gene Expression Analysis
  • DNA/Protein Synthesis and Degradation Analysis
  • Protein-Protein, Protein-DNA, Protein-RNA Interaction Analysis
  • Protein Post-Translational Modification and Activation Analysis
  • Customized Services – Please contact us for your special needs.