Cell Engineering and Cell-Based Assays
- Gene knock-out, allele-specific genome editing, and inducible gene knock-down
- Drug resistant cell line generation, stable cell line generation, phenotypic analysis (CRISPR/Cas9, lentiviral system)
- Cell Proliferation Assays (growth rate, doubling time, viability)
- Cell Death Assays (apoptosis, necrosis, autophagy)
- Cell Cycle Assays (cell cycle phases, mitosis, quiescence, senescence)
- Subcellular Localization & Translocation Assays (endocytosis, exocytosis, nuclear/cytosol)
- Subcellular Organelle Functional Assays (mitochondria, Golgi, endoplasmic reticulum)
- Cell Motility, Invasion, and Chemotaxis Assay
- Customized Services – Please contact us for your special needs
CRISPR/Cas9 Based Method for KO/KI Cell Line Generation
Target-based Mechanistic Assays
- DNA, RNA, protein level
- Subcellular localization
- Signaling pathway research
- Cellular detection of protein phosphorylation
- Protein-Protein interaction
- Cell metabolite detection
- Cell morphology and phenotype analysis
Potency Correlation of Protein-Protein Interaction
Cell Function
- Cell viability/toxicity
- Cell proliferation
- Cell cycle
- Cell motility/migration
- Cell apoptosis
- 2D/3D Clonogenic assay
OrisTM Migration Assay
Transwell Migration Assay
3D Clonogenic Assay
Outstanding Platform to Enable Cancer Immunotherapy with One-Stop Service
- Immunological function assessment
- Tumor microenvironment analysis
- In vivo efficacy evaluation on animal models
- NGS to understand TCR/BCR repertoire
- Patient TILs analysis
Immuno-oncology In Vitro Functional Assays
- Immune cell activation assays
- T/B/NK cell activation
- Macrophage differentiation assays
- M1/M2 macrophage differentiation
- DC differentiation
- MDSC induction
- T cell polarization assays
- Treg/Th1/Th2/Th17 differentiation
- T cell cytotoxicity assay
- Cytotoxic T lymphocyte assay (OT-1 system)
- Mixed lymphocyte reaction
- Primary immune cell profiling
- Immune cell suppressive assay
- MDSC/Treg/Tumor-T suppressive assay
- Antibody mediated immunological assay
- ADCC/CDC/ADCP
Assay | WuXi capibility (Human/Mouse) | |
Call activation | T cell | Yes (H/M) |
B cell | Yes (H) | |
NK cell | Yes (H) | |
Cell differentiation/ polarization | M1/M2 macrophage | Yes (H/M) |
MDSC | Yes (H) | |
DC | Yes (H/M) | |
Treg, Th1, Th2, Th17 polarization | Yes (H) | |
Mixed Lymphocyte Reaction | DC co-culture with T | Yes (H/M) |
Suppressive Assay | MDSC suppressive assay | Yes (H) |
Treg suppressive assay | Yes (H) | |
Tumor cell+immune cell co-culture | Yes (H) | |
Cell cytoxicity | Cytotoxic T lymphocyte assay (OT-1 system) | Yes (M) |
NK cytotoxicity | Yes (H) | |
ADCC, ADCP | Yes (H) | |
CDC | Yes (H) | |
Cell exhaustion | T cell exhaustion | Yes (M) |
Immuno-oncology In Vitro Technical Competence
Immune cell profiling in tumor infiltrating leukocyte (TIL) and other tissues (spleen, blood, lymph node, liver, etc.) profiling:
- Immune cell population analysis (T, B, NK, DC, Myeloid, Macrophage, Granulocyte, CD4+T, CD8+T, Treg, Th1/2/17, naïve/memory/effector T, g/mMDSC, M1/M2, etc.)
- Functional and intracellular marker analysis (PD-1, PD-L1/2, CTLA-4, TIM-3, LAG-3, OX40, 4-1BB, ICOS, Ki-67, IL-2, IL-17, IFNγ, TNFα, Granzyme B, etc.)
Biomarker
- Soluble biomarker – ELISA, ELISPOT
- Cell-based biomarker – FACS
- Tissue-based biomarker – Multiplex IHC
- Gene expression profiling-RNAseq, Nanostring
Tumor Infiltrating Lymphocytes (TILs) Analysis on Human and Murine Tumor Tissues
- CD4, CD8, MDSC, M1/M2, DC cell, NK cell, B cell
- PD1, TIM3, LAG3, OX40, 4-1BB
- Treg, Th1,2,3,17 …
In Vivo Efficacy Evaluation on Animal Models Syngeneic Murine Models
- Immune-checkpoint humanized models
- Immune-Avatar humanized models PBMC humanized models
- HSC humanized models
TM (Tumor Microenvironment) Analysis for Immunoscore by FACS, IHC and IF
CTLs, NK, Tregs, MDSC, TAM, etc.
Lymphoid Tissue and Peripheral Blood Analysis
- Immune cell populations and subsets – frequency, activation, and differentiation
- Cytokine and chemokine analysis
- Anatomical localization ofin vivo anti-tumor immune responses
High Content Platform
Overview
Cell-based functional bioassays of bio-relevant activities of enzymes, receptors, and signaling molecules and pathways. Integrated multi-parameter analysis for customized specific needs.
Applications
- Targeted Drug Screening
- Target-Hit Search
- Hit to Lead Identification
- Lead Optimization
- Drug Mechanism of Actions
- Drug Kinetics
- Drug Cytotoxicity
Specific Assays: 2D/3D/4D Image Based Quantitative and Semi-Quantitative Functional Bioassays
- Cell Proliferation Assay (growth rate, doubling time, viability, etc.)
- Cell Death Assay (apoptosis, necrosis, autophagy)
- Cell Cycle Assay (cell cycle phases, mitosis, quiescence, senescence)
- Subcellular Localization & Translocation Assay (endocytosis, exocytosis, nuclear/cytosol, nuclear structure)
- Subcellular Organelle Function Assay (mitochondria, Golgi body, endoplasmic reticulum, etc.)
- Membrane Integrity and Function Analysis
- Kinase and Signal Transduction Analysis
- Cell-Cell Interaction Analysis
- Cell Sub-Population Analysis and Enrichment
- Cytoskeleton and Extracellular Matrix Analysis
- Cell Motility, Invasion, and Chemotaxis Assay
- Oxidative Stress and Cellular Response Analysis
- DNA and Chromosome Damage, and Genetic Instability Analysis
- Epigenetic Modification Analysis
- Transcription Activation and Gene Expression Analysis
- DNA/Protein Synthesis and Degradation Analysis
- Protein-Protein, Protein-DNA, Protein-RNA Interaction Analysis
- Protein Post-Translational Modification and Activation Analysis
- Customized Services – Please contact us for your special needs.