Cellular Assays
Cell Engineering Service
- Gene knock-out, Allele-specific genome editing, Inducible gene knock-down
- Drug resistant cell line generation, Stable cell line generation
- Phenotypic analysis (Key technology: CRISPR/Cas9, Lentiviral system)
CRISPR/Cas9 Based Method for KO/KI Cell Line Generation
Cellular Assays
- Cell Proliferation Assays (growth rate, doubling time, viability)
- Cell Death Assays (apoptosis, necrosis, autophagy)
- Cell Cycle Assays (cell cycle phases, mitosis, quiescence, senescence)
- Subcellular Localization & Translocation Assays (endocytosis, exocytosis, nuclear/cytosol)
- Subcellular Organelle Functional Assays (mitochondria, Golgi, endoplasmic reticulum)
- Cell Motility, Invasion, and Chemotaxis Assay
Target-based Mechanistic Assays
- DNA, RNA, protein level
- Subcellular localization
- Signaling pathway research
- Cellular detection of protein phosphorylation
- Protein-Protein interaction
- Cell metabolite detection
- Cell morphology and phenotype analysis
Potency Correlation of Protein-Protein Interaction
Cell Function
- Cell viability/toxicity
- Cell proliferation
- Cell cycle
- Cell motility/migration
- Cell apoptosis
- 2D/3D Clonogenic assay
Transwell Migration Assay
3D Clonogenic Assay
OrisTM Migration Assay
