Cell Engineering Service

  • Gene knock-out, Allele-specific genome editing, Inducible gene knock-down
  • Drug resistant cell line generation, Stable cell line generation
  • Phenotypic analysis (Key technology: CRISPR/Cas9, Lentiviral system)

CRISPR/Cas9 Based Method for KO/KI Cell Line Generation

CRISPR/Cas9 Based Method for KO/KI Cell Line Generation, sgRNA design, stable cell line, gene knock-out, in

Cellular Assays

  • Cell Proliferation Assays (growth rate, doubling time, viability)
  • Cell Death Assays (apoptosis, necrosis, autophagy)
  • Cell Cycle Assays (cell cycle phases, mitosis, quiescence, senescence)
  • Subcellular Localization & Translocation Assays (endocytosis, exocytosis, nuclear/cytosol)
  • Subcellular Organelle Functional Assays (mitochondria, Golgi, endoplasmic reticulum)
  • Cell Motility, Invasion, and Chemotaxis Assay

Target-based Mechanistic Assays

  • DNA, RNA, protein level
  • Subcellular localization
  • Signaling pathway research
  • Cellular detection of protein phosphorylation
  • Protein-Protein interaction
  • Cell metabolite detection
  • Cell morphology and phenotype analysis

Potency Correlation of Protein-Protein Interaction

Potency Correlation of Protein-Protein Interaction, binding assays, IC50 determination

Cell Function

  • Cell viability/toxicity
  • Cell proliferation
  • Cell cycle
  • Cell motility/migration
  • Cell apoptosis
  • 2D/3D Clonogenic assay

Transwell Migration Assay

Transwell Migration Assay, cell motility and cell cycle

3D Clonogenic Assay

3D Clonogenic Assay, cell proliferation, toxicity, soft agar assay geltrex

OrisTM Migration Assay