CRISPR/Cas9 Technology
CRISPR/Cas9 genome editing system becomes a powerful tool to target and modify genomic sequence, allowing a previously unattainable level of efficacy, specificity and efficiency. This system uses non-coding RNAs to guide the Cas9 nuclease to generate sequence-specific DNA cleavage of target loci across the genome. WuXi Biology has fully implemented this technology platform to offer CRISPR/Cas9 based gene knock-in or knock-out to support gene function studies and target validation efforts.
- CRISPR/Cas9 Applications:
- Specific gene knock-out
- Precise genome editing, such as site-mutation knock-in or add reporter gene tag
- Rapid generation of genetically engineered mice
- Genome-wide scale knock-out for screening
- Key Advantages of CRISPR/Cas9:
- Simple and fast two-component system with high efficiency and low cost
- Permanently turn off genes at the DNA level; clean background
- Offers access to non-coding regions of the genome
CRISPR/Cas9 Gene Editing Services
- sgRNA design, plasmid construction, and validation
- Knock-out of single or multiple target sequences
- Site-mutation knock-in and gene tag knock-in
- Isogenic cell line generation with gene knock-out/knock-in
- Genome-wide CRISPR/Cas9 library screen
CRISPR in vivo services for model generation and rare genetic disease
- From genome editing to disease models and PK-PD-efficacy studies
- Well-established in vivo gene editing platform in rodent
- 100+ KO and KI mouse/rat lines generated
- Generation of genetically engineered animal models (GEAM) for drug discovery for rare genetic disease
- Knockout and knock in services in mouse and rat
- Humanization: in situ replace mouse gene with matched human exons or introns
- Transgenic, genotyping, and phenotyping
- In vitro fertilization
- Embryo and sperm cryopreservation
- Drug administration, animal and tissue manipulation and histopathology
- AAALAC accreditation and GLP compliance