Phage Display

Phage display technology fuses the peptide-coding gene with the phage coat protein gene, allowing peptides to be displayed on the surface of the phage. Its amplifiability enables successive rounds of biopanning to enrich low-abundance but high-affinity sequences from the original library. WuXi Biology offers diverse screening strategies and display longer and structurally complex peptides.

Features

  • Natural amino acids dominate
  • Compatible with cellular selection and in vivo selection
  • Panning format:  Protein based panning (Protein captured by beads or coated on the surface of tube), cell based panning

Off-The-Shelf Peptide Phage Libraries

WuXi provides Off-The-Shelf Peptide Phage Libraries with flexible length

  • 5 monocyclic peptide libraries (15 AA)
  • 3 linear peptide libraries (9 AA, 12 AA, 30 AA)

Average diversity: 8E+09

Average diversity: 4E+09

Customized Peptide Phage Libraries

Codons can also be optimized for customized library based on two strategies for oligonucleotide synthesis. WuXi Biology  has the ability of the whole workflow of phage library construction from pilot test of ligation and transfection to large scale ligation and transfection for library construction.

Phage-Display Panning and Screening Workflow

WuXi Biology provides panning services and hits validation. In addition to conventional ELISA, we have newly introduced a high-throughput sequencing-based approach to final more candidates.

Case Study – Antigen X Panning Against C7C Cyclic Peptide Library

  • Binding affinity determination of two synthetic peptides using titration ELISA and SPR
  • The affinities of 2 peptides in further SPR data are consistent with ELISA, both less than 100nM