mRNA Display

mRNA display is a powerful technique that leverages the natural process of translation to generate and screen large libraries of peptides, offering a unique blend of high diversity, flexibility, and efficiency. This method involves the in vitro translation of an mRNA library. WuXi Biology provides End-to-End services ranging from library synthesis and screening to post-screening validation.

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Features

  • Incorporation of limited unnatural AAs
  • Creation of libraries with greater diversity

mRNA Display Libraries

WuXi Biology offers both off-the-shelf libraries and custom synthesis libraries. If you have additional ideas, our scientists can collaborate with you to design new libraries.

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mRNA Display Workflow

WuXi Biology provides screening services and post-screening validation, with a comprehensive delivery report to uncover key patterns in big data from multiple perspectives.

Case Study – mRNA Display Validation Results

• Binding affinity determination of 2 pipetides using SPR

• Both orthosteric and allosteric binders were discovered

• Hit rate is close to 60% (KD <20 μM)

• Using SPR, we then identified the cyclization site through cysteine-to-serine mutations and employed alanine scanning to identify five essential binding residues in peptide B

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