mRNA Services Platform

Design and optimization of mRNA sequences
- Utilizing the design and optimization techniques for mRNA sequences, UTR screening, and codon optimization
Plasmid and DNA template productions for in vitro transcription
- Employment of a classical three-step purification method
- Implementation of mono-enzyme digestion and purification through chromatography
Research scale mRNA production and characterization
- Generation of conventional 5’ capped mRNA with chemically modified nucleosides (optional: a two-step method)
- Analysis of purity, integrity, capping efficiency, poly A length and dsRNA residue, and endotoxins residue

mRNA delivery
- LNP (microfluidic system) and others
  ✓ Tissue targeted LNP
  ✓ SORT strategy
- Lipoplexes (LPX)
Characterizations
- Particle size distribution
- Polydispersity index (PDI)
- Zeta potential
- pKa
- Encapsulation efficiency
- Particle morphology
- Endotoxin residue

In vitro and in vivo delivery and biodistribution

Cell lines: over 1500 cell lines and patient-derived fibroblasts, establishment of
customized stable cell lines
Primary cells: primary hepatocytes of human, mouse, rat, dog and monkey;
availability of other customized primary cells
Animal models: various species of rodents and NHP
Methods for evaluations of mRNA uptake and protein expression: bDNA,
LC/MS, HCS, ELISA , Western blot, flow cytometry, IF and more
In vivo delivery and biodistribution: live image system.