mRNA Services Platform

Design and production of mRNA

• Design and optimization of mRNA sequences
  • Utilizing the design and optimization techniques for mRNA sequences, UTR screening, and codon optimization
• Plasmid and DNA template productions for in vitro transcription
  • Employment of a classical three-step purification method
  • Implementation of mono-enzyme digestion and purification through chromatography
• Research scale mRNA production and characterization
  • Generation of conventional 5’ capped mRNA with chemically modified nucleosides (optional: a two-step method)
  • Analysis of purity, integrity, capping efficiency, poly A length and dsRNA residue, and endotoxins residue

mRNA delivery system

• mRNA delivery
  • LNP (microfluidic system) and others
    ✓ Tissue targeted LNP
    ✓ SORT strategy
  • Lipoplexes (LPX)
• Characterizations
  • Particle size distribution
  • Polydispersity index (PDI)
  • Zeta potential
  • pKa
  • Encapsulation efficiency
  • Particle morphology
  • Endotoxin residue

In vitro and in vivo evaluations

In vitro and in vivo delivery and biodistribution

• Cell lines: over 1500 cell lines and patient-derived fibroblasts, establishment of
  customized stable cell lines
• Primary cells: primary hepatocytes of human, mouse, rat, dog and monkey;
  availability of other customized primary cells
• Animal models: various species of rodents and NHP
• Methods for evaluations of mRNA uptake and protein expression: bDNA,
  LC/MS, HCS, ELISA , Western blot, flow cytometry, IF and more
• In vivo delivery and biodistribution: live image system.

Flyer_Supporting Discovery of mRNA Drugs

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