Mouse hind limb tumor metastatic model to evaluate prophylaxis and treatment
We have established and optimized a robust tumor bone metastasis mouse model, which recapitulates the tumor metastasis progression from circulation to bone colonization, and provides a window for evaluating prophylaxis and treatment.
Specifically, we inoculated two breast cancer cell lines, JIMT-1 and MDA-MB-231, each with high metastatic potential, into the mouse intra-caudal artery. By detection of a steadily incremental bioluminescence signal, a prophylaxis window was observed to precede metastases, which were found exclusively on mouse hind limbs. These mice exhibited swollen hind limbs and even paralysis at a late stage. Gross necropsy and pathology analysis confirmed bone localization, the invasion and spreading of tumor cells to muscles and bone marrow, and the lesions in tibias and femurs. This robust bone metastases model is able to imitate the multiple stages of tumor metastasis, leading to exclusive bone metastasis and thereby providing for an evaluation of prophylaxis and treatment.
7 Bone metastasis model_6010_Yao